pAAV-DJ-N589X
(Plasmid #130878)

• Purpose: Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon
• Depositing Lab: Prashant Mali
• Publication: Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.1038/s41598-018-21742-x.

Backbone

• Vector backbone: Unknown
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 7333
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AAV-DJ cap N589X
• Species: AAV
• Insert Size (bp): 2214
• Mutation: N589X
• Promoter: Unknown

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Orignial plasmid was purchased from Vector Biolabs. However, the mutations have been introduced in house.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry