pAAV2-N587X
(Plasmid
#130877)
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PurposeEncodes the AAV2 capsid with the N587 residue replaces with a TAG stop codon
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnknown
- Backbone size w/o insert (bp) 5100
- Total vector size (bp) 7327
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAAV2 cap N587X
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SpeciesAAV
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Insert Size (bp)2208
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MutationN587X
- Promoter Unknown
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOrignial plasmid was purchased from Vector Biolabs. However, the mutations have been introduced in house.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV2-N587X was a gift from Prashant Mali (Addgene plasmid # 130877 ; http://n2t.net/addgene:130877 ; RRID:Addgene_130877) -
For your References section:
Oligonucleotide conjugated multi-functional adeno-associated viruses. Katrekar D, Moreno AM, Chen G, Worlikar A, Mali P. Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.1038/s41598-018-21742-x. 10.1038/s41598-018-21742-x PubMed 29483550
