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PurposeIntegration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneColE1
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Vector typeExpression of mKate, leu2(Q180*), and Ura3 on orthogonal plasmid after integration onto p1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemKate
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FDP-P10B2-A75-RZ-URA3 was a gift from Chang Liu (Addgene plasmid # 130874 ; http://n2t.net/addgene:130874 ; RRID:Addgene_130874) -
For your References section:
Tunable Expression Systems for Orthogonal DNA Replication. Zhong Z, Ravikumar A, Liu CC. ACS Synth Biol. 2018 Dec 21;7(12):2930-2934. doi: 10.1021/acssynbio.8b00400. Epub 2018 Nov 16. 10.1021/acssynbio.8b00400 PubMed 30408954
