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PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCEN/ARS
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTP-DNAP1
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SpeciesK. lactis pGKL1/2 system
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MutationL477V, L640Y, I777K, W814N
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAR-Ec633 was a gift from Chang Liu (Addgene plasmid # 130873 ; http://n2t.net/addgene:130873 ; RRID:Addgene_130873) -
For your References section:
Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds. Ravikumar A, Arzumanyan GA, Obadi MKA, Javanpour AA, Liu CC. Cell. 2018 Dec 13;175(7):1946-1957.e13. doi: 10.1016/j.cell.2018.10.021. Epub 2018 Nov 8. 10.1016/j.cell.2018.10.021 PubMed 30415839