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Addgene

mTLR4 flag Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 13087 with NotI-HF and SalI-HF.

We expect and see the following banding patterns:

NotI-HF single: 1 band: ~7.2kb
NotI-HF / SalI-HF double: 2 bands: ~2.5 and 4.7kb