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Addgene

mTLR4 flag
(Plasmid #13087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag-CMV-1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLR4
  • Alt name
    toll-like receptor 4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Mutation
    Amino acids 1-19 have been replaced with mammalian signal sequence (SALLILALVGAAVA) followed by Flag tag
  • GenBank ID
    NM_021297
  • Entrez Gene
    Tlr4 (a.k.a. Lps, Ly87, Ran/M1, Rasl2-8)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV30
  • 3′ sequencing primer CMV24
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTLR4 flag was a gift from Ruslan Medzhitov (Addgene plasmid # 13087 ; http://n2t.net/addgene:13087 ; RRID:Addgene_13087)
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