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Addgene

MLKS2delKASHexp
(Plasmid #130862)

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Item Catalog # Description Quantity Price (USD)
Plasmid 130862 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pH7WG2
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MLKS2
  • Species
    Zea mays (maize)
  • Mutation
    Deletion of KASH domain
  • Tag / Fusion Protein
    • eGFP-FLAG-HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLKS2delKASHexp was a gift from Hank Bass (Addgene plasmid # 130862 ; http://n2t.net/addgene:130862 ; RRID:Addgene_130862)

  • For your References section:

    MLKS2 is an ARM domain and F-actin-associated KASH protein that functions in stomatal complex development and meiotic chromosome segregation. Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann K, Bass HW. Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795. 10.1080/19491034.2019.1629795 PubMed 31221013
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