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Addgene

MLKP1delKASHec
(Plasmid #130845)

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Item Catalog # Description Quantity Price (USD)
Plasmid 130845 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLKP1
  • Species
    Zea mays (maize)
  • Mutation
    Deletion of KASH domain, addition of three alanine residues
  • Tag / Fusion Protein
    • eGFP-FLAG-HA (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLKP1delKASHec was a gift from Hank Bass (Addgene plasmid # 130845 ; http://n2t.net/addgene:130845 ; RRID:Addgene_130845)

  • For your References section:

    Identification and characterization of genes encoding the nuclear envelope LINC complex in the monocot species Zea mays. Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW. J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 10.1242/jcs.221390. 10.1242/jcs.221390 PubMed 30659121
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