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Addgene

pAG470 LifeAct-iFAST
(Plasmid #130821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone size w/o insert (bp) 3969
  • Total vector size (bp) 4446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct-iFAST
  • Alt name
    LifeAct-FAST2
  • Alt name
    iFAST fused to LifeAct
  • Alt name
    FAST2 fused to LifeAct
  • Species
    Synthetic
  • Insert Size (bp)
    477
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG470 LifeAct-iFAST was a gift from Arnaud Gautier (Addgene plasmid # 130821 ; http://n2t.net/addgene:130821 ; RRID:Addgene_130821)

  • For your References section:

    Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging. Benaissa H, Ounoughi K, Aujard I, Fischer E, Goiame R, Nguyen J, Tebo AG, Li C, Le Saux T, Bertolin G, Tramier M, Danglot L, Pietrancosta N, Morin X, Jullien L, Gautier A. Nat Commun. 2021 Nov 30;12(1):6989. doi: 10.1038/s41467-021-27334-0. 10.1038/s41467-021-27334-0 PubMed 34848727
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