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Addgene

hTLR1 flag
(Plasmid #13081)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13081 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFlag-CMV-1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLR1
  • Alt name
    toll-like receptor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    Amino acids 1-18 have been replaced with mammalian signal sequence (SALLILALVGAAVA) followed by Flag tag
  • GenBank ID
    NM_003263 AAY85640.1
  • Entrez Gene
    TLR1 (a.k.a. CD281, TIL, TIL. LPRS5, rsc786)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV30
  • 3′ sequencing primer CMV24
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate that GenBank ID AAY85640.1 is better match for the human TLR1 insert sequence in this plasmid. When compared to GenBank ID NM_003263 there is an apparent mutation (R80T) which may represent a valid polymorphism.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hTLR1 flag was a gift from Ruslan Medzhitov (Addgene plasmid # 13081 ; http://n2t.net/addgene:13081 ; RRID:Addgene_13081)