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Addgene

pMSCVbsd-PPARD-L432A
(Plasmid #130797)

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Item Catalog # Description Quantity Price (USD)
Plasmid 130797 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMSCVbsd
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPARD L432A
  • Species
    H. sapiens (human)
  • Mutation
    L432A
  • Entrez Gene
    PPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pMSCVseqfw (CCCTTGAACCTCCTCGTTCGACC)
  • 3′ sequencing primer pMSCVseqrv (GAGACGTGCTACTTCCATTTGTC)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generated by PCR mutagenesis. Please visit https://www.biorxiv.org/content/10.1101/525576v2 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVbsd-PPARD-L432A was a gift from Till Adhikary (Addgene plasmid # 130797 ; http://n2t.net/addgene:130797 ; RRID:Addgene_130797)

  • For your References section:

    PPARbeta/delta recruits NCOR and regulates transcription reinitiation of ANGPTL4. Legrand N, Bretscher CL, Zielke S, Wilke B, Daude M, Fritz B, Diederich WE, Adhikary T. Nucleic Acids Res. 2019 Oct 10;47(18):9573-9591. doi: 10.1093/nar/gkz685. 10.1093/nar/gkz685 PubMed 31428774
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