pKM200
(Plasmid #13079)

• Depositing Lab: Kenan Murphy
• Publication: Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Backbone

• Vector backbone: pMAK705
• Backbone size w/o insert (bp): 5700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid contains temperature sensitive origin of replication and must be grown at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ptac-gam-bet-exo operon
• Alt name: Lambda Red
• Species: bacteriophage lambda
• Insert Size (bp): 2000
• GenBank ID: J02459

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anthony Poteete UMass Medical School
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ptac-gam-bet-exo operon derived from plasmid pTP806