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pKM201
(Plasmid #13078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAMPts
  • Backbone manufacturer
    Greg Phillips
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid contains temperature sensitive origin of replication and must be grown at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ptac-gam-bet-exo operon
  • Alt name
    Lambda Red
  • Species
    bacteriophage lambda
  • Insert Size (bp)
    2000
  • GenBank ID
    J02459

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Anthony Poteete UMass Medical School

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ptac-gam-bet-exo operon derived from plasmid pTP806

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM201 was a gift from Kenan Murphy (Addgene plasmid # 13078 ; http://n2t.net/addgene:13078 ; RRID:Addgene_13078)

  • For your References section:

    Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy KC, Campellone KG. BMC Mol Biol. 2003 Dec 13. 4():11. 10.1186/1471-2199-4-11 PubMed 14672541
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