pKM208
(Plasmid #13077)

• Depositing Lab: Kenan Murphy
• Publication: Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Backbone

• Vector backbone: pKM201
• Backbone manufacturer: Kenan Murphy
• Backbone size w/o insert (bp): 7200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid contains temperature sensitive origin of replication and must be grown at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: lacI repressor
• Species: E. coli
• Insert Size (bp): 1400
• GenBank ID: J01636
• Entrez Gene: lacI (a.k.a. b0345, ECK0342)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anthony Poteete UMass Medical School
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

lacI gene obtained from plasmid pTP550