362 pCS Cherry DEST
(Plasmid #13075)

• Depositing Lab: Nathan Lawson
• Publication: Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 5778

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Must be grown in a strain resistant to ccdB.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cherry
• Insert Size (bp): 752

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cherry is from Roger Tsien.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a H30R mutation in mCherry. This mutation does not affect plasmid function and the construct has been validated for expression.