pET51b(+)_SNAP-p24
(Plasmid #130718)

• Purpose: Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein
• Depositing Lab: Kai Johnsson
• Publication: Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7.

Backbone

• Vector backbone: pET51-b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5218
• Total vector size (bp): 6433
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SNAP-Tag-p24
• Alt name: P24 HIV Capsid protein fused to SNAP-Tag
• Species: Synthetic
• Insert Size (bp): 1371
• Mutation: Deleted first Met, Ala and Last Ser from the Enhancer nanobody
• Tags / Fusion Proteins:
  • Hisx10 (C terminal on insert)
  • StrepTag (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pET51b(_)_SNAP-p24.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry