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pET51b(+)_LAMA-N95 (for GFP)
(Plasmid #130716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51-b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5218
  • Total vector size (bp) 5952
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LAMA-N95
  • Alt name
    cpDHFRL24G5 inserted into the enhancer nanobody at N95
  • Species
    Synthetic
  • Insert Size (bp)
    888
  • Mutation
    Deleted first Met, Ala and Last Ser from the Enhancer nanobody
  • Tag / Fusion Protein
    • Hisx10 (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b(+)_LAMA-N95 (for GFP) was a gift from Kai Johnsson (Addgene plasmid # 130716 ; http://n2t.net/addgene:130716 ; RRID:Addgene_130716)

  • For your References section:

    Chemogenetic Control of Nanobodies. Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. 10.1038/s41592-020-0746-7 PubMed 32066961
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