pCDNA3.0_LynLAMA-F98 IRES EGFP
(Plasmid
#130710)
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PurposePlasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.0
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 8218
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn11-SNAP-Tag-LAMA-F98 IRES EGFP
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Alt namecpDHFRL24G5 inserted into the enhancer nanobody at F98 and EGFP
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SpeciesSynthetic
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Insert Size (bp)2839
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Tags
/ Fusion Proteins
- Lyn11 (N terminal on insert)
- SNAP-Tag (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.0_LynLAMA-F98 IRES EGFP was a gift from Kai Johnsson (Addgene plasmid # 130710 ; http://n2t.net/addgene:130710 ; RRID:Addgene_130710) -
For your References section:
Chemogenetic Control of Nanobodies. Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. 10.1038/s41592-020-0746-7 PubMed 32066961