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pCDNA3.0_mitoLAMA-F98
(Plasmid #130704)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6889
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NTOM20-SNAP-Tag-LAMA-F98
  • Alt name
    cpDHFRL24G5 inserted into the enhancer nanobody at F98
  • Species
    Synthetic
  • Insert Size (bp)
    1503
  • Tags / Fusion Proteins
    • NTOM20 (N terminal on insert)
    • SNAP-Tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.0_mitoLAMA-F98 was a gift from Kai Johnsson (Addgene plasmid # 130704 ; http://n2t.net/addgene:130704 ; RRID:Addgene_130704)

  • For your References section:

    Chemogenetic Control of Nanobodies. Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. 10.1038/s41592-020-0746-7 PubMed 32066961
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