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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 5818
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsMust be grown in a strain resistant to ccdB.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6x Myc Tag
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Insert Size (bp)260
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
221 pCS 3MT DEST was a gift from Nathan Lawson (Addgene plasmid # 13070 ; http://n2t.net/addgene:13070 ; RRID:Addgene_13070) -
For your References section:
Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311