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Addgene

pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG
(Plasmid #130680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 7866
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMCHD1 Exon1-36.47M-48
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4770
  • Mutation
    D1523_G1960del
  • Entrez Gene
    SMCHD1 (a.k.a. BAMS, FSHD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG was a gift from Stephen Tapscott (Addgene plasmid # 130680 ; http://n2t.net/addgene:130680 ; RRID:Addgene_130680)

  • For your References section:

    Identification of SMCHD1 domains for nuclear localization, homo-dimerization, and protein cleavage. Hiramuki Y, Tapscott SJ. Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z. 10.1186/s13395-018-0172-z PubMed 30071896
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