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pUTKan-3xHA-GFP-TOM5
(Plasmid #130674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUTKan
  • Backbone manufacturer
    Karin Schumacher
  • Backbone size w/o insert (bp) 10862
  • Total vector size (bp) 11826
  • Vector type
    Plant Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Translocase of outer mitochondrial membrane 5
  • Alt name
    TOM5
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    166
  • Entrez Gene
    TOM5 (a.k.a. AT5G08040, T22D6.2, mitochondrial import receptor subunit TOM5 homolog)
  • Promoter Ubiquitin10
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • sGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer CGATTTTCTGGGTTTGATCG
  • 3′ sequencing primer aagaccggcaacaggattc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/672972v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUTKan-3xHA-GFP-TOM5 was a gift from Andreas Weber (Addgene plasmid # 130674 ; http://n2t.net/addgene:130674 ; RRID:Addgene_130674)

  • For your References section:

    Rapid Single-Step Affinity Purification of HA-Tagged Plant Mitochondria. Kuhnert F, Stefanski A, Overbeck N, Drews L, Reichert AS, Stuhler K, Weber APM. Plant Physiol. 2020 Feb;182(2):692-706. doi: 10.1104/pp.19.00732. Epub 2019 Dec 9. 10.1104/pp.19.00732 PubMed 31818904
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