360 pCR kdrb
(Plasmid
#13067)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3900
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekdrb
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SpeciesD. rerio (zebrafish)
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Entrez Genekdr (a.k.a. flk1, flk1b, kdrb, si:busm1-205d10.1, si:ch211-254j6.1, si:ch211-278f21.4, vegfr, vegfr2b, wu:fc31a09)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 rev
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For riboprobe, digest with NotI and use Sp6 polymerase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
360 pCR kdrb was a gift from Nathan Lawson (Addgene plasmid # 13067 ; http://n2t.net/addgene:13067 ; RRID:Addgene_13067) -
For your References section:
Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish. Covassin LD, Villefranc JA, Kacergis MC, Weinstein BM, Lawson ND. Proc Natl Acad Sci U S A. 2006 Apr 25. 103(17):6554-9. 10.1073/pnas.0506886103 PubMed 16617120