ppk301-T2A-QF2 HDR plasmid
(Plasmid #130667)

• Purpose: Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene
• Depositing Lab: Leslie Vosshall
• Publication: Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.

Backbone

• Vector backbone: pUC57
• Vector type: donor template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
• Species: Synthetic; Neurospora crassa, Discosoma sp., Aedes aegypti
• Insert Size (bp): 2569

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • ppk301-T2A-QF2.gb
• A portion of this plasmid was derived from a plasmid made by: QF2 was obtained from Chris Potter (e.g. Addgene plasmid #104876)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry