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Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
(Plasmid #130665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMos-3xP3-dsRed
  • Backbone size w/o insert (bp) 8200
  • Total vector size (bp) 11980
  • Modifications to backbone
    removed 3xP3-dsRed marker by PCR
  • Vector type
    Insect Expression ; Ae. aegypti expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
  • Species
    M. musculus (mouse), Synthetic; Aedes aegypti
  • Insert Size (bp)
    3780
  • Promoter Aedes aegypti poly-ubiquitin

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGAAACAGCTATGACC
  • 3′ sequencing primer GCAATAGCATCACAAATTTCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pub-mCD8:GFP-T2A-DsRed:NLS-SV40 was a gift from Leslie Vosshall (Addgene plasmid # 130665 ; http://n2t.net/addgene:130665 ; RRID:Addgene_130665)

  • For your References section:

    The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti. Matthews BJ, Younger MA, Vosshall LB. eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963. 10.7554/eLife.43963 PubMed 31112133
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