pSL0376 (His-MBP-StrepII-TniQ)
(Plasmid
#130641)
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PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2CT-10
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Vector typeBacterial Expression, CRISPR ; Transposon
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVchTniQ
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SpeciesVibrio cholerae
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GenBank IDWP_000479715.1
- Promoter T7
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Tag
/ Fusion Protein
- His10-MBP-TEVsite-StrepII on TniQ (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Previously referred to as INTEGRATE.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL0376 (His-MBP-StrepII-TniQ) was a gift from Samuel H. Sternberg (Addgene plasmid # 130641 ; http://n2t.net/addgene:130641 ; RRID:Addgene_130641) -
For your References section:
Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH. Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. Epub 2019 Jun 12. 10.1038/s41586-019-1323-z PubMed 31189177