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Addgene

pSL0374 (His-MBP-Cas8_Cas7_Cas6)
(Plasmid #130639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2CT-10
  • Vector type
    Bacterial Expression, CRISPR ; Transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VchCas8, VchCas7, VchCas6
  • Species
    Vibrio cholerae
  • GenBank ID
    WP_001194012.1, WP_000775488.1, WP_000872476.1
  • Promoter T7
  • Tag / Fusion Protein
    • His10-MBP-TEVsite on Cas8 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Previously referred to as INTEGRATE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL0374 (His-MBP-Cas8_Cas7_Cas6) was a gift from Samuel H. Sternberg (Addgene plasmid # 130639 ; http://n2t.net/addgene:130639 ; RRID:Addgene_130639)

  • For your References section:

    Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH. Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. Epub 2019 Jun 12. 10.1038/s41586-019-1323-z PubMed 31189177
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