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PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDFDuet-1
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Vector typeBacterial Expression, CRISPR ; Transposon
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
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SpeciesVibrio cholerae
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GenBank IDWP_000479715.1, WP_001194012.1, WP_000775488.1, WP_000872476.1
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Previously referred to as INTEGRATE.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL0284 (pQCascade_entry) was a gift from Samuel H. Sternberg (Addgene plasmid # 130635 ; http://n2t.net/addgene:130635 ; RRID:Addgene_130635) -
For your References section:
Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH. Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. Epub 2019 Jun 12. 10.1038/s41586-019-1323-z PubMed 31189177
