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Addgene

pSL0283 (pTnsABC)
(Plasmid #130633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCOLADuet-1
  • Vector type
    Bacterial Expression, CRISPR ; Transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VchTnsA, VchTnsB, VchTnsC
  • Species
    Vibrio cholerae
  • GenBank ID
    WP_000202732.1, WP_001074608.1, WP_002024152.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Previously referred to as INTEGRATE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL0283 (pTnsABC) was a gift from Samuel H. Sternberg (Addgene plasmid # 130633 ; http://n2t.net/addgene:130633 ; RRID:Addgene_130633)

  • For your References section:

    Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH. Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. Epub 2019 Jun 12. 10.1038/s41586-019-1323-z PubMed 31189177
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