MoChlo: Modular Cloning Chloroplast Toolbox
(Kit #
1000000156
)
Depositing Lab: Scott Lenaghan
The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. This kit includes a library of 115 level-0 modules (47 promoters, 36 5’UTRs, 9 promoter-5’UTR fusions, 10 3’UTRs and 13 genes of interest) along with 7 level-2 destination vectors specific for either tobacco (Nicotiana tabacum) or potato (Solanum tuberosum).
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Occhialini A, Piatek AA, Pfotenhauer AC, Frazier TP, Stewart CN Jr, Lenaghan SC. Plant Physiology. 2019 Mar; 179(3):943-957. doi: 10.1104/pp.18.01220. PubMed PMID: 30679266.
Description
The MoChlo Toolbox is a standardized library of chloroplast-specific parts to allow fast and flexible design of synthetic operons for chloroplast transformation and metabolic engineering in plants. Based on Golden-Gate cloning and compatible with previous plant MoClo systems, the MoChlo Toolbox includes a library of 115 level-0 modules (47 promoters, 36 5’UTRs, 9 promoter-5’UTR fusions, 10 3’UTRs and 13 genes of interest) along with 7 level-2 destination vectors specific for either tobacco (Nicotiana tabacum) or potato (Solanum tuberosum).
Schematic representation of the Modular Cloning Chloroplast Toolbox (MoChlo) for assembly of chloroplast-specific transformation vectors. (A) Standardized chloroplast-specific Level-0 modules; (B) Level-1 assembly of individual expression cassettes (from 1 to 7); (C) Level-2 assembly of a synthetic operon integrated into a chloroplast transformation vector.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The MoChlo: Modular Cloning Chloroplast Toolbox was a gift from Scott Lenaghan (Addgene kit #1000000156).”
For your Reference section:
MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Occhialini A, Piatek AA, Pfotenhauer AC, Frazier TP, Stewart CN Jr, Lenaghan SC. Plant Physiology. 2019 Mar; 179(3):943-957. doi: 10.1104/pp.18.01220. PubMed PMID: 30679266.
MoChlo: Modular Cloning Chloroplast Toolbox - #1000000156
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin |