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Draetta Mini-Human AsCpf1-Based Human Genome-Wide Knockout Library
(Pooled Library #130630)

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  • Purpose

    This lentiviral genome-wide knockout library targets 16,977 human genes. Each gene is targeted by an AsCpf1(AsCas12a)-based array containing 3–4 guides concatenated in one vector.

  • Vector Backbone

    LentiUniversal-Puro (Plasmid #127749) - does not express AsCpf1(AsCas12a)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 130630 Mini-Human AsCpf1-Based Human Genome-Wide KO Library† 1 $325 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with LentiAsCpf1-3xMYC-blast (Addgene #127750) or any other plasmid(s) or cell lines expressing AsCpf1(AsCas12a).

Library Details

  • Species
    Human
  • Genes targeted
    16,977
  • Guide arrays
    17,032
  • Controls
    55 non-targeting arrays
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mini-human AsCpf1-based Human Genome-wide Knockout Library was a gift from Giulio Draetta (Addgene #130630)

  • For your References section:

    Pooled library screening with multiplexed Cpf1 library. Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G. Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. PubMed 31316073
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