Skip to main content
Addgene

27 pCR plcg 1 CDS
(Plasmid #13058)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    plcg1
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    plcg1 (a.k.a. fj46f04, wu:fj46f04, wu:fj57a05)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS.

For riboprobe, digest with MluI and use SP6 polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    27 pCR plcg 1 CDS was a gift from Nathan Lawson (Addgene plasmid # 13058 ; http://n2t.net/addgene:13058 ; RRID:Addgene_13058)

  • For your References section:

    phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson ND, Mugford JW, Diamond BA, Weinstein BM. Genes Dev. 2003 Jun 1. 17(11):1346-51. 10.1101/gad.1072203 PubMed 12782653
Related items: