TP997
(Bacterial strain #13055)

• Depositing Lab: Anthony Poteete
• Publication: Poteete et al BioTechniques. 2006 Sept. 41(3):261-264

Backbone

• Vector backbone: na
• Vector type: Bacterial Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Spectinomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: TP997 is a bacterial strain and does not contain a plasmid. It grows at 37 degrees.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: aacC1
• Species: Tn1696
• Insert Size (bp): 600

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that TP997 is NOT a plasmid, but a bacterial strain. It does not contain a plasmid.

TP997 is a strain containing a synthetic operon with the aacC1 gene (gentamicin resistance) fused to an artificial ribosome binding sequence and a variant of the synthetic promoter CP6. Strain genotype: MG1655 lacIPdelta::bla-aadA1148 galK::aacC1067.