pcDNA3 V5 hHR23A
(Plasmid #13053)

• Depositing Lab: Steven Grossman
• Publication: Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Backbone

• Vector backbone: pcDNA3.1D V5 His TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hHR23A
• Alt name: HR23A
• Alt name: Rad23a
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Entrez Gene: RAD23A (a.k.a. HHR23A, HR23A)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

You can cut out the hHR23A insert with HindIII and XhoI.