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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1D V5 His TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehHR23A
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Alt nameHR23A
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Alt nameRad23a
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Entrez GeneRAD23A (a.k.a. HHR23A, HR23A)
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
You can cut out the hHR23A insert with HindIII and XhoI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 V5 hHR23A was a gift from Steven Grossman (Addgene plasmid # 13053 ; http://n2t.net/addgene:13053 ; RRID:Addgene_13053) -
For your References section:
A post-ubiquitination role for MDM2 and hHR23A in the p53 degradation pathway. Brignone C, Bradley KE, Kisselev AF, Grossman SR. Oncogene. 2004 May 20. 23(23):4121-9. 10.1038/sj.onc.1207540 PubMed 15064742