DsRed-Rab5 DN
(Plasmid #13051)

• Depositing Lab: Richard Pagano
• Publication: Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72.

Backbone

• Vector backbone: pDsRed2-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAB5A (Canis familiaris)
• Alt name: DsRed-rab5 dominant negative
• Species: Canis familiaris
• Insert Size (bp): 699
• Mutation: asparagine 3 changed to serine (neutral); asparagine 133 changed to isoleucine (dominant negative); serine 210 changed to asparagine (neutral)
• GenBank ID: NM_001003317
• Entrez Gene: RAB5A
• Tags / Fusion Proteins:
  • DsRed2 (N terminal on backbone)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: DsRed1-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the modified canine Rab5A was received from Cecilia Bucci (University of Lecce, Lecce, Italy)
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry