pET23MutS-F39A
(Plasmid
#13044)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET23
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMutS
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SpeciesT. aquaticus
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Insert Size (bp)3000
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MutationPhe39Ala mutation Full length
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GenBank IDU33117
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from pETMutS
Biswas, Il and Hsieh,P.
1996 JBC 271:5040-5048
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23MutS-F39A was a gift from Peggy Hsieh (Addgene plasmid # 13044 ; http://n2t.net/addgene:13044 ; RRID:Addgene_13044) -
For your References section:
Photocross-linking of the NH2-terminal region of Taq MutS protein to the major groove of a heteroduplex DNA. Malkov VA, Biswas I, Camerini-Otero RD, Hsieh P. J Biol Chem. 1997 Sep 19. 272(38):23811-7. 10.1074/jbc.272.38.23811 PubMed 9295328