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Addgene

pETMutSdelta768
(Plasmid #13041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen(Stratagene)
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 (DE3)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MutS
  • Species
    T. aquaticus
  • Insert Size (bp)
    2300
  • Mutation
    deleted C-terminal aa residues 769 - 811
  • GenBank ID
    U33117

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETMutSdelta768 was a gift from Peggy Hsieh (Addgene plasmid # 13041 ; http://n2t.net/addgene:13041 ; RRID:Addgene_13041)

  • For your References section:

    Disruption of the helix-u-turn-helix motif of MutS protein: loss of subunit dimerization, mismatch binding and ATP hydrolysis. Biswas I, Obmolova G, Takahashi M, Herr A, Newman MA, Yang W, Hsieh P. J Mol Biol. 2001 Jan 26. 305(4):805-16. 10.1006/jmbi.2000.4367 PubMed 11162093
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