EGFP-supervillin
(Plasmid #13040)

• Depositing Lab: Elizabeth Luna
• Publication: Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36. doi: 10.1242/jcs.112.13.2125

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: supervillin
• Alt name: p205
• Species: B. taurus (bovine)
• Insert Size (bp): 6000
• GenBank ID: AF025996
• Entrez Gene: SVIL
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains cDNA from nucleotides -80 to 5420. Endogenous ClaI converted to KpnI for 5' cloning site.