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Addgene

pBluescript GPX-1
(Plasmid #13038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPX-1
  • Alt name
    GPX1
  • Alt name
    Glutathione Peroxidase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    856
  • Mutation
    Additional GCG upstream of codon 11. Codon 92 is CTG.
  • Entrez Gene
    GPX1 (a.k.a. GPXD, GSHPX1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fong Fong Chu, City of Hope, Duarte, CA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript GPX-1 was a gift from Peter Newburger (Addgene plasmid # 13038 ; http://n2t.net/addgene:13038 ; RRID:Addgene_13038)

  • For your References section:

    Sequences in the 3'-untranslated region of the human cellular glutathione peroxidase gene are necessary and sufficient for selenocysteine incorporation at the UGA codon. Shen Q, Chu FF, Newburger PE. J Biol Chem. 1993 May 25. 268(15):11463-9. PubMed 7684384
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