Skip to main content
Addgene

pRSET Nucleolin
(Plasmid #13037)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Preferred growth strain is BL21(DE3) for high protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nucleolin
  • Alt name
    C23
  • Alt name
    FLJ 45706
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • GenBank ID
    NM_005381
  • Entrez Gene
    NCL (a.k.a. C23, Nsr1)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET Nucleolin was a gift from Peter Newburger (Addgene plasmid # 13037 ; http://n2t.net/addgene:13037 ; RRID:Addgene_13037)

  • For your References section:

    Recognition and binding of the human selenocysteine insertion sequence by nucleolin. Wu R, Shen Q, Newburger PE. J Cell Biochem. 2000 Apr . 77(3):507-16. 10.1002/(SICI)1097-4644(20000601)77:3<507::AID-JCB15>3.0.CO;2-P PubMed 10760958
Related items:
Commonly requested with: