pKM154 Citations (7)
Originally described in: PCR-mediated gene replacement in Escherichia coli.Murphy KC, Campellone KG, Poteete AR Gene. 2000 Apr 4. 246(1-2):321-30. PubMed Journal
Articles Citing pKM154
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Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli. Zerbini F, Zanella I, Fraccascia D, Konig E, Irene C, Frattini LF, Tomasi M, Fantappie L, Ganfini L, Caproni E, Parri M, Grandi A, Grandi G. Microb Cell Fact. 2017 Apr 24;16(1):68. doi: 10.1186/s12934-017-0681-1. PubMed |
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH.
Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x.
PubMed
Associated Plasmids |
Improved sgRNA design in bacteria via genome-wide activity profiling.
Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH.
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
PubMed
Associated Plasmids |
Dynamics of transcription-translation coordination tune bacterial indole signaling. Wang T, Zheng X, Ji H, Wang TL, Xing XH, Zhang C. Nat Chem Biol. 2020 Apr;16(4):440-449. doi: 10.1038/s41589-019-0430-3. Epub 2019 Dec 23. PubMed |
Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding. Specht DA, Xu Y, Lambert G. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11274-11282. doi: 10.1073/pnas.1918685117. Epub 2020 May 6. PubMed |
Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells. Feng H, Guo J, Wang T, Zhang C, Xing XH. Nucleic Acids Res. 2021 Feb 22;49(3):1263-1277. doi: 10.1093/nar/gkaa1295. PubMed |
Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli. Konig E, Zerbini F, Zanella I, Fraccascia D, Grandi G. Bio Protoc. 2018 Jan 20;8(2):e2688. doi: 10.21769/BioProtoc.2688. eCollection 2018 Jan 20. PubMed |
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