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Addgene

pKM154
(Plasmid #13036)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTP809
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cat, sacB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM154 was a gift from Kenan Murphy (Addgene plasmid # 13036 ; http://n2t.net/addgene:13036 ; RRID:Addgene_13036)

  • For your References section:

    PCR-mediated gene replacement in Escherichia coli. Murphy KC, Campellone KG, Poteete AR. Gene. 2000 Apr 4. 246(1-2):321-30. 10.1016/S0378-1119(00)00071-8 PubMed 10767554
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