Skip to main content
Addgene

pRSET YBX-1
(Plasmid #13035)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Preferred growth strain is BL21(DE3) for high protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YBX1
  • Alt name
    Y Box Binding Protein 1
  • Alt name
    DBPB
  • Alt name
    NSEP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1481
  • GenBank ID
    NM_004559
  • Entrez Gene
    YBX1 (a.k.a. BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET YBX-1 was a gift from Peter Newburger (Addgene plasmid # 13035 ; http://n2t.net/addgene:13035 ; RRID:Addgene_13035)

  • For your References section:

    Identification and molecular cloning of a human selenocysteine insertion sequence-binding protein. A bifunctional role for DNA-binding protein B. Shen Q, Wu R, Leonard JL, Newburger PE. J Biol Chem. 1998 Mar 6. 273(10):5443-6. 10.1074/jbc.273.10.5443 PubMed 9488664
Related items:
Commonly requested with: