pcDNA3-ps-CFP
(Plasmid #13034)

• Purpose: Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence
• Depositing Lab: Doug Golenbock
• Publication: Fluorescent Protein Plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: photo-switchable cyan fluorescent protein
• Alt name: PS-CFP
• Alt name: PS-CFP2
• Species: H. sapiens (human)
• Insert Size (bp): 717
• GenBank ID: AY533824
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ps-CFP is cloned from pPS-CFP2-N from Evrogen.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The EXFP-R primer (5'-GTCTTGTAGTTGCCGTCGTC-3') can be used to sequence GFP based constructs to differentiate EGFP vs ECFP vs EYFP.