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Addgene

pGL3-ELAM-luc
(Plasmid #13029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ELAM
  • Alt name
    ELAM-1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SELE (a.k.a. CD62E, ELAM, ELAM1, ESEL, LECAM2, selectin-e)
  • Tag / Fusion Protein
    • Luc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ??? (unknown if destroyed)
  • 3′ cloning site ??? (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer lucNrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-ELAM-luc was a gift from Doug Golenbock (Addgene plasmid # 13029 ; http://n2t.net/addgene:13029 ; RRID:Addgene_13029)
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