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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
(Plasmid #130275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD132.102 (pmyo-2)
  • Backbone manufacturer
    Fire Lab (Addgene plasmid #1662)
  • Backbone size w/o insert (bp) 4156
  • Total vector size (bp) 5001
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arch(D95N)
  • Species
    Halorubrum sodomense
  • Insert Size (bp)
    774
  • Tag / Fusion Protein
    • 2x Myc-tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNH11 (pmyo-2::Arch(D95N)::2xMycTag) was a gift from Alexander Gottschalk (Addgene plasmid # 130275 ; http://n2t.net/addgene:130275 ; RRID:Addgene_130275)

  • For your References section:

    Rhodopsin-based voltage imaging tools for use in muscles and neurons of Caenorhabditis elegans. Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, Bach M, Liewald JF, Gottschalk A. Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 10.1073/pnas.1902443116. 10.1073/pnas.1902443116 PubMed 31371514
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