pNH13 (pmyo-2::QuasAr::mOrange)
(Plasmid
#130273)
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PurposeExpresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130273 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD132.102 (pmyo-2)
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Backbone manufacturerFire Lab (Addgene plasmid #1662)
- Backbone size w/o insert (bp) 4187
- Total vector size (bp) 5692
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQuasAr::mOrange
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SpeciesHalorubrum sodomense
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Insert Size (bp)1505
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Tag
/ Fusion Protein
- mOrange (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH13 (pmyo-2::QuasAr::mOrange) was a gift from Alexander Gottschalk (Addgene plasmid # 130273 ; http://n2t.net/addgene:130273 ; RRID:Addgene_130273) -
For your References section:
Rhodopsin-based voltage imaging tools for use in muscles and neurons of Caenorhabditis elegans. Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, Bach M, Liewald JF, Gottschalk A. Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1902443116. doi: 10.1073/pnas.1902443116. 10.1073/pnas.1902443116 PubMed 31371514
