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Addgene

pJPF0190
(Plasmid #130268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 7600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRKACA
  • Alt name
    Protein Kinase A Catalytic Subunit alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1053
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Promoter CAG
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

https://www.addgene.org/23495/ is where PRKACA was originally cloned from.

Please visit https://www.biorxiv.org/content/10.1101/747030v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJPF0190 was a gift from Hana El-Samad (Addgene plasmid # 130268 ; http://n2t.net/addgene:130268 ; RRID:Addgene_130268)

  • For your References section:

    Biphasic Response of Protein Kinase A to Cyclic Adenosine Monophosphate Triggers Distinct Epithelial Phenotypes. Fonseca JP, Weinberg ZY, Aslankoohi E, El-Samad H. bioRxiv 2020, 747030 10.1101/747030
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