pJPF0117
(Plasmid #130267)

• Purpose: Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP
• Depositing Lab: Hana El-Samad
• Publication: Fonseca et al bioRxiv 2020, 747030

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 7500
• Total vector size (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: bPAC
• Alt name: Bacterial photo-activable adenylyl cyclase
• Species: Beggiatoa
• Insert Size (bp): 1059
• Promoter: hPGK
• Tag / Fusion Protein:
  • tagBFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • jpf0117_plenti-pgk-tagbfp-nls-t2a-bpac-nes-puro.gb
• A portion of this plasmid was derived from a plasmid made by: https://www.addgene.org/103017/ is where bPAC was originally cloned from
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://www.addgene.org/103017/ is where bPAC was originally cloned from.

Please visit https://www.biorxiv.org/content/10.1101/747030v2 for bioRxiv preprint.