pRDAX-EMMI-010 BsaI//(A)-Esp3I-SacB-Esp3I-(Z)//BsaI
(Plasmid
#130218)
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PurposeEMMA Kit # 1000000119 extension
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecustom built
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSacB
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Speciesbacterial
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRDAX-EMMI-010 BsaI//(A)-Esp3I-SacB-Esp3I-(Z)//BsaI was a gift from Gary Lewin (Addgene plasmid # 130218 ; http://n2t.net/addgene:130218 ; RRID:Addgene_130218)